Indian Journal of Biotechnology

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Published by: NISCAIR (CSIR)

Subjects: Biology

Indian Journal of Biotechnology, started as a quarterly journal in 2002, publishes full papers, short communications and reviews in agricultural, animal, environmental, industrial, medical, and microbial biotechnology, bioinformatics, and socio-legal and ethical aspects in biotechnology. The latest developments in biotech-industry are covered under Notes and News.

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National Institute of Science Communication and Information Resources
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Phone: 25841846 / 25846304-07
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E-mail: ijbt@niscair.res.in


ISSN No. :    0972-5849
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Production, efficient recovery and partial characterization of biodegradable polymer produced by soil Streptomyces sp.
Title: Production, efficient recovery and partial characterization of biodegradable polymer produced by soil Streptomyces sp. Authors: Wani, S J; Sayyed, R Z Abstract: In the present paper, we report the production of poly-b-hydroxybutyrate (PHB) by local soil isolates of Streptomyces sp. under submerged fermentation in nitrogen deficient medium. Streptomyces sp. was isolated on glycerol asparagine medium from soil samples collected from the campus of PSGVP Mandal, Shahada (Maharashtra) India. Of the seven isolates of Streptomyces sp., two isolates, namely, RZS 1 and RZS 2, accumulated 1.7 g/L (4.52 g/L biomass) and 1.5 g/L (4.11 g/L biomass) PHB, respectively on the basis of dry wt. Further increase in intracellular level of PHB was observed during the growth of these isolates in carbon rich medium with pH 7 at 30oC. We also report the use of modified solvent system consisting of 1:1 mixture of acetone and ethanol as a more specific and efficient recovery method vis-à-vis traditionally used sodium hypochlorite system. Description: 127-129

Rapid typing of Pasteurella multocida isolates from haemorrhagic septicaemia cases by multiplex PCR for their virulence
Title: Rapid typing of Pasteurella multocida isolates from haemorrhagic septicaemia cases by multiplex PCR for their virulence Authors: Somshekhar, S H; Veeregowda, B M; Suryanarayana, V V S; Basawaraj, Awati; Isloor, S; Rathnamma, D Abstract: A total of 12 P. multocida isolates recovered from field outbreaks of haemorrhagic septicaemia in buffaloes were studied for the presence of 4 virulence genes (pfhA, tbpA, hgbB & toxA) in comparison with vaccine strain by multiplex PCR. The study showed the presence of pfhA and tbpA in all the field isolates and vaccine strain. None of the isolates harboured toxA and hgbB. The virulence genes tbPA and pfhA appear to be closely related to bovine P. multocida serogroup B isolates and are important for severe form of the disease. Description: 124-126

Assessment of genetic fidelity in in vitro regenerated plants of Brassica juncea (L.) Czern & Coss. using RAPD and ISSR markers
Title: Assessment of genetic fidelity in in vitro regenerated plants of Brassica juncea (L.) Czern & Coss. using RAPD and ISSR markers Authors: Verma, V; Thakur, A K; Singh, B K; Chauhan, D K; Singh, K H; Chauhan, J S Abstract: RAPD and ISSR markers were used to assess the genetic integrity of Indian mustard var. NRCDR-2 plants regenerated from cotyledon explants via direct organogenesis. Of 60 (40 RAPD & 20 ISSR) markers screened, only 27 RAPD and 12 ISSR markers produced a total of 286 (198 RAPD and 88 ISSR) clear, distinct and reproducible amplicons. All the RAPD markers produced a total of 198 bands, ranging 50 to 8000 bp length and the number of scorable bands varied 3 to 15 per primer with an average of 7.33 bands per primer. Whereas, all the ISSRs produced a total of 88 amplicons in the size range of 300 to 3000 bp and 4 to 12 scorable bands per primer with an average of 7.33 bands per primer. Analysis of RAPD and ISSR banding patterns generated by PCR amplification gave zero evidence for the presence of somaclonal variations. The amplified products were monomorphic across all the regenerated plants and were similar to the mother plant. This homogenous amplification-profile confirmed the clonal fidelity of the tissue culture raised Indian mustard plants. Description: 120-123

Morphological and molecular descriptors for first Indian mustard hybrid, NRCHB 506
Title: Morphological and molecular descriptors for first Indian mustard hybrid, NRCHB 506 Authors: Thakur, A K; Singh, K H; Singh, Lal; Verma, Vandana; Singh, Dhiraj Abstract: Characterization of the first Indian mustard hybrid, NRCHB 506 and its parental lines MJA 5 and MJR 1 had been carried out using morphological traits and molecular markers. Hybrid and its parents expressed distinctness for five morphological traits. Hybrid NRCHB 506 could be distinguished from MJA 5 on the basis of number of seeds/siliqua; being very few (13-≤16) in MJA 5 but medium (17-≤20) in the hybrid. The hybrid NRCHB 506 was alike MJA 5 for remaining four characteristics, namely, number of lobes in leaf, width of petals in flower, plant height and 1000-seed wt; while it was distinct from MJR 1 for these four traits,. During molecular characterization studies, 255 SSR markers, derived from Brassica rapa, B. nigra, B. napus, B. carinata, B. oleracea and Arabidopsis thaliana, were used. Finally, for genetic analysis, two SSR markers, viz., SJ-34121 and Ni4G06, were found to be the most suitable for hybridity confirmation and purity assessment of hybrid seeds. Marker SJ-34121 identified male specific 330 bp repeat and female specific 270 bp repeat; while Ni4G06 also detected one male and one female specific repeat of 110 and 165 bp, respectively in the hybrid. Description: 116-119

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