Indian Journal of Biotechnology

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Published by: NISCAIR (CSIR)

Subjects: Biology

Indian Journal of Biotechnology, started as a quarterly journal in 2002, publishes full papers, short communications and reviews in agricultural, animal, environmental, industrial, medical, and microbial biotechnology, bioinformatics, and socio-legal and ethical aspects in biotechnology. The latest developments in biotech-industry are covered under Notes and News.

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ISSN No. :    0972-5849
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Molecular characterization and genetic diversity assessment in soybean [Glycine max (L.) Merr.] varieties using SSR markers
Title: Molecular characterization and genetic diversity assessment in soybean [Glycine max (L.) Merr.] varieties using SSR markers

Authors: Chauhan, Devendra K; Bhat, J A; Thakur, A K; Kumari, S; Hussain, Z; Satyawathi, C T

Abstract: Soybean varieties (48) released from different breeding stations belonging to different agro-climatic zones were characterized with 21 SSR markers with at least one primer from each linkage group. The total number of alleles detected by the aforesaid primers was 84 with a maximum and minimum frequency of 7 and 2, respectively. The maximum and minimum PIC values were found to be 0.781and 0.304 for the primers SOY HSP176 and Satt278, respectively. The average genetic similarity coefficient from all possible combinations was found to be 0.267. The genetic similarity coefficient for most number of pairs was in the range of 0.1-0.4, indicating high diversity amongst the chosen genotypes. All the 48 varieties could be easily distinguished from each other based on the selected markers and thus were effective for varietal identification and diversity analysis of soybean genotypes. A dendrogram based on STMS loci profile showed good agreement with their cultivars pedigree information.

Page(s): 504-510

Assessment of genetic fidelity in somaclonal variants with cytological and RAPD analysis in Oroxylum indicum (L.) Vent.—An endangered medicinal tree
Title: Assessment of genetic fidelity in somaclonal variants with cytological and RAPD analysis in Oroxylum indicum (L.) Vent.—An endangered medicinal tree

Authors: Gokhale, Mamta; Bansal, Y K

Abstract: Random amplified polymorphic DNA (RAPD) markers were used to verify the clonal fidelity in in vitro regenerated plants of Oroxylum indicum (L.) Vent. (Bignoniaceae). Deviation from normal/natural leaf shape and branching pattern was detected in indirectly (from callus) regenerated plants. Percentage of phenotypical variation was observed and calculated upto five subculture passages. RAPD profiles of directly (from axillary bud) and indirectly regenerated plants were compared with mother plant. To screen the genetic variation 20 arbitrary decamers were used to study of randomly selected nature grown plant as well as direct and indirect regenarants. Genetic variation among nature grown and direct regenerants was 10%, whereas 24.59% polymorphism frequency was estimated for indirect plantlets. These results indicate that the regeneration of plants through axillary bud culture is a low-risk method for generating genetic variability, whereas material regenerated through callus has genetic variation, resulting in the formation of somaclones. Cytological studies of cells of callus, root tip of directly and indirectly regenerated plants showed the incidence of polyploidy.

Page(s): 581-584

In vitro seed germination and plant regeneration of an epiphytic orchid Aerides ringens (Lindl.) Fischer
Title: In vitro seed germination and plant regeneration of an epiphytic orchid Aerides ringens (Lindl.) Fischer

Authors: Srivastava, Deepti; Gayatri, M C; Sarangi, S K

Abstract: Aerides ringens (Lindl.) Fischer is an epiphytic and endemic orchid of Western Ghats, India, which is popular for its beautiful flowers and fox-tail inflorescence. The present study offers novel and reproducible protocol for in vitro seed germination of A. ringens. Seed viability was determined by triphenyl tetrazolium chloride and fluorescein diacetate staining methods and 88.86%±6.7 seeds were found viable at the time of inoculation. Viable seeds were then evaluated for germination on Murashige and Skoog (MS) basal medium and Knudson C (KC) medium supplemented with variable concentrations of 6-benzyl amino purine (BAP) and peptone. Seed culture on KC medium supplemented with 4.44 μM BAP and 500 mg L-1 peptone exhibited best rate of seed germination (89.28±3.42%), which transformed into green colour vigorous and compact protocorms of 1.89±0.38 mm size. Protocorms were subcultured on KC medium fortified with different concentrations and combination of cytokinins [BAP, kinetin (KN) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (thidiazuron; TDZ) and auxin (IAA)]. Best results were obtained in the presence of Kn (9.3 μM), which produced 4.40±2.20 shoots per segment of 3.05±0.46 cm length. Seedlings were transferred to KC medium fortified with auxins (IAA, NAA & IBA) for rooting. Half strength KC with IAA (5.71 μM) produced strong and stout 4.44±1.61 roots of 3.22±0.40 cm length per plantlet. Harden and acclimatized plants were established ex vitro with 80% survival rate.

Page(s): 574-580

Effect of explant origin on clonal propagation of Aegle marmelos (L.) Corr. —A multipurpose tree species
Title: Effect of explant origin on clonal propagation of Aegle marmelos (L.) Corr. —A multipurpose tree species

Authors: Parveen, Shahina; Shahzad, Anwar; Nazim, Farah

Abstract: A comparative analysis was performed on the clonal propagation from aerial nodal segments and root sucker derived nodal segments of Aegle marmelos (L.) Corr. on MS medium supplemented with various concentrations of plant growth regulators (PGRs). The results proved that the nodal segments from root suckers have great potentials to multiply rapidly and produced large number of shoots (12.4±0.7) compared to aerial nodal segments (5.8±0.6) on a similar medium (MS+BA 5.0 µM+NAA 0.5 µM) and culture conditions (6 wk). Cultures were maintained up to the 5th subculture stage on the optimal regeneration medium for further multiplication and proliferation and by the end of 3rd subculture passage, a maximum of 40.4±3.6 shoots/explant were achieved from root sucker derived nodal segments. Ex vitro rooting in microshoots was employed to shorten the propagation span, with an added effect on acclimatization success. During acclimatization, estimation of different photosynthetic pigments was also carried out.

Page(s): 566-573

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