Indian Journal of Biotechnology

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Published by: NISCAIR (CSIR)

Subjects: Biology

Indian Journal of Biotechnology, started as a quarterly journal in 2002, publishes full papers, short communications and reviews in agricultural, animal, environmental, industrial, medical, and microbial biotechnology, bioinformatics, and socio-legal and ethical aspects in biotechnology. The latest developments in biotech-industry are covered under Notes and News.

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ISSN No. :    0972-5849
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Efficient Pseudomonas putida for degradation of p-nitrophenol
Title: Efficient Pseudomonas putida for degradation of p-nitrophenol

Authors: Kulkarni, Meenal; Chaudhari, Ambalal

Abstract: Exploration of Pseudomonas putida for controlling environmental (p-nitrophenol) PNP pollution revealed that 0.5 OD inoculum at 600 nm degraded 100 ppm of PNP completely under the ambient conditions within 24 h as established by UV-visible spectrophotometric and TLC analysis of treated broth and confirmed the same by HPLC analysis. An attempt to elucidate the mechanism of PNP degradation indicated that (i) it was oxidative with a stoichiometric release of nitrite, (ii) PNP was converted to hydroquinone, which accumulated in the presence of 2,2' -dipyridyl, (iii) hydroquinone was the first product of ring oxygenation and nitrite removal, as confirmed by TLC and HPLC analysis and (iv) concomitant formation of a keto acid by ring fission, which was confirmed by Rothera's test.  

Page(s): 411-415

Molecular genetic architecture of Hallikar cattle (Bos indicus) revealed through microsatellite markers
Title: Molecular genetic architecture of Hallikar cattle (Bos indicus) revealed through microsatellite markers

Authors: Hepsibha, P; Gogoi, A; Karthickeyan, S M K

Abstract: Hallikar breed of cattle, popularly known as the ‘champion of draft breeds’, is a typical Mysore type cattle. Molecular characterization of Hallikar cattle through automated genotyping of 22 microsatellite loci revealed a total of 166 alleles with the number of alleles ranging from 3 (ILSTS033) to 16 (TGLA53) with a mean of 7.55±0.65, and the mean effective number of alleles was 3.67±0.31. The sizes of the alleles ranged from 92 (CSRM60) to 302 bp (ILSTS006). The overall mean polymorphic information content value was 0.6367±0.03. The observed (Ho) and expected heterozygosity (He) values averaged 0.6068±0.05 and 0.6863±0.03, respectively, measuring high genetic variation in the population. The overall mean deficit of heterozygotes (FIS) (0.1314±0.05) exhibited existence of a breed structure in Hallikar.

Page(s): 411-413

Effect of heparin-binding oviduct specific proteins on in vitro embryo development in cattle
Title: Effect of heparin-binding oviduct specific proteins on in vitro embryo development in cattle

Authors: Sharma, Aditya K; Mohapatra, Sushil K; Mohanty, A K; Das, Subrata K

Abstract: The aim of the present study was to evaluate the effect of exogenous heparin-binding oviduct specific proteins (HBP) on in vitro embryo development in cattle. Total protein (TP) was precipitated from cow oviduct tissue lysate by ammonium sulfate and fractionated by heparin agarose affinity column. SDS-PAGE profile of TP showed diverse molecular size bands. HBP showed bands between ~66 to ~97 kDa and unbound protein fraction (HUBP) exhibited ~45 to ~66 kDa bands. Protein fractions (TP, HBP & HUBP) were used in 3 different concentrations (1, 10 & 30 µg/mL) on in vitro embryo development. Cumulus-oocyte complexes were collected from slaughterhouse ovaries, washed 4-5 times and cultured in maturation media at 38.5°C with maximum humidity in 5% CO2 in air. After 24 h, oocytes were co-incubated with capacitated sperms in Fert-BO for 14-18 h and then transferred into embryo development media. Cleavage was checked after 40-42 h and transferred into replacement media. The results indicate that at 10 µg/mL of TP fraction used as media supplement increased the cleavage rate (71.87%±3.78, P<0.05) and blastocyst formation (29.37%±3.13, P<0.01) as compared to HBP and HUBP fraction. From this study, it can be concluded that both TP and HBP may play a major role during the process of fertilization and early embryonic development in vitro.

Page(s): 408-410

Revisiting the role of the electron transport chain in molybdate reduction by Enterobacter cloacae strain 48
Title: Revisiting the role of the electron transport chain in molybdate reduction by Enterobacter cloacae strain 48

Authors: Shukor, Mohd Yunus

Abstract: Molybdenum reduction to molybdenum blue by microbes is a potential bioremediation tool for molybdenum pollution. A previous work using cyanide as a respiratory inhibitor has shown that the site of molybdenum reduction in Enterobacter cloacae strain 48 is at the electron transport pathway. In the present work, several respiratory inhibitors together with cyanide were used to reinvestigate the site of molybdenum reduction in E. cloacae strain 48. All the respiratory inhibitors tested showed no inhibition to the molybdenum-reducing capability of the bacterium. It was also discovered that cyanide caused a large increase in the pH of the enzymatic reaction mixture and, therefore, the inhibition previously seen was likely due to unfavourable pH for the enzyme activity. Based on these results, the site of molybdenum reduction in this bacterium is suggested not to be the component of electron transport pathway.

Page(s): 404-407

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